Phalaenopsis from seed.

Discussion in 'Propagation This Month' started by hydrogardener, Apr 21, 2018.

  1. CarolineL

    CarolineL Super Gardener

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    Hi @hydrogardener do you have more details of how you set up your sterile conditions please? I have always wanted to try this but the things I read made it look a massive amount of work, and needing supplies that are not readily available in hobby quantities
     
  2. hydrogardener

    hydrogardener Super Gardener

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    There is a post on my blog regarding how I started the orchid seeds.

    Hydroponic Workshop: April 2018

    Also, there are a number of sites and videos on youtube if you search for starting orchid seeds at home.

    I used a pressure cooker, but some people use a microwave and get results. Instead of a laminar flow hood I used a clear tote from Wal-Mart. Everything is sprayed with 90% Isopropyl Alcohol to to sterilize it. The tools and vessels must be sterile. The media I purchased on Ebay and it is not expensive. I tried making my own but found it cheaper to just buy it on Ebay.

    If you have questions send me a message and I will see if I can help.

    Good luck.
     
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    • hydrogardener

      hydrogardener Super Gardener

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      I crossed it with a miniature plant with all purple flowers so the hybrid should be interesting.
       
    • hydrogardener

      hydrogardener Super Gardener

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      They are almost ready to leave the nest.

      Phalaenopsis bambino.jpg
       
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      • longk

        longk Total Gardener

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        That is very impressive! Well done :blue thumb:
         
      • CarolineL

        CarolineL Super Gardener

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        Yes, seriously impressive! Have you ended up growing on thousands? :snorky:
         
      • hydrogardener

        hydrogardener Super Gardener

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        I hardly have room for one let alone thousands! A few of the larger more vigorous plants will be grown on, and even those will probably be given away. There is another plant with seed pods that I intend to work with. The pods from that plant contain seeds that were cross-pollinated last spring. The parents are a large and a miniature phalaenopsis. I will surely keep one of those plants if successful.
         
      • CarolineL

        CarolineL Super Gardener

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        Ooo @hydrogardener - that is very controlled of you. I'd always be afraid that the one I kept turned out rubbish, and the ones I discarded could have been fantastic - with hybrids you never know...
         
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        • hydrogardener

          hydrogardener Super Gardener

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          Today I decided to deflask one of the phalaenopsis seedlings to see if they are ready to live outside of the flasks. The seedling was planted in sphagnum moss moistened with a dilute nutrient solution and will be initially grown in a domed container then gradually be acclimated to life outside the dome.

          Deflasked phalaenopsis seedling.jpg

          incubator.jpg
           
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            Last edited: Nov 2, 2018
          • hydrogardener

            hydrogardener Super Gardener

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            As my test plant is doing nicely I decided to deflask one more plant. Then, I saw another promising plant I just could not throw away, then another, and another and so on, until at eight I decided enough is enough. I will ask my wife to get rid of the excess plants before I have a house full of them. :rolleyespink:

            eight babies.jpg
             
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            • longk

              longk Total Gardener

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              Careful - a woman can decide that all plants are excess if the mood takes her :heehee:
               
            • CarolineL

              CarolineL Super Gardener

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              I told you that there'd be a temptation to keep more!
               
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              • Sirius

                Sirius Total Gardener

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                Interesting project.
                Well done on getting some seedlings.

                I have considered growing fern spore in vitro.
                But I think it must be difficult to have everything 100% contaminant free - particularly the spore themselves.
                 
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                • hydrogardener

                  hydrogardener Super Gardener

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                  It would seem to be the same as orchid seeds. They were sterilized by soaking them in a syringe with hydrogen peroxide and then using the syringe to place them on the media under sterile conditions. The syringe came from a pharmacy and is used to administer medicine to babies by mouth, there is no needle involved.

                  My loss to contamination was much less than expected, less than 20% I would estimate.
                   
                  Last edited: Nov 12, 2018
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